Ontology highlight
ABSTRACT:
SUBMITTER: Paschon DE
PROVIDER: S-EPMC6408524 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Nature communications 20190308 1
Genome editing for therapeutic applications often requires cleavage within a narrow sequence window. Here, to enable such high-precision targeting with zinc-finger nucleases (ZFNs), we have developed an expanded set of architectures that collectively increase the configurational options available for design by a factor of 64. These new architectures feature the functional attachment of the FokI cleavage domain to the amino terminus of one or both zinc-finger proteins (ZFPs) in the ZFN dimer, as ...[more]