Ontology highlight
ABSTRACT:
SUBMITTER: Lei C
PROVIDER: S-EPMC3005779 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20101101 11
We have successfully expressed recombinant mitochondrial-type ferredoxin (mtFd) and ferredoxin:NADP(+) reductase (mtFNR) from Cryptosporidium parvum and characterized their biochemical features for the first time for an apicomplexan. Both C. parvum mtFd (CpmtFd) and FNR (CpmtFNR) were obtained and purified as holo-proteins, in which the correct assembly of [2Fe-2S] cluster in Fd and that of FAD in FNR were confirmed and characterized by UV/vis and electron paramagnetic resonance. These proteins ...[more]