Ontology highlight
ABSTRACT:
SUBMITTER: Labay V
PROVIDER: S-EPMC3005947 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Labay Valentina V Weichert Rachel M RM Makishima Tomoko T Griffith Andrew J AJ
Biochemistry 20100908 39
Mutations of transmembrane channel-like gene 1 (TMC1) cause hearing loss in humans and mice. TMC1 is the founding member of a family of genes encoding proteins of unknown function that are predicted to contain multiple transmembrane domains. The goal of our study was to define the topology of mouse TMC1 expressed heterologously in tissue culture cells. TMC1 was retained in the endoplasmic reticulum (ER) membrane of five tissue culture cell lines that we tested. We used anti-TMC1 and anti-HA anti ...[more]