Adenosine and inosine release during hypoxia in the isolated spinal cord of neonatal rats.

ABSTRACT:

Background and purpose

Adenosine and inosine accumulate extracellularly during hypoxia/ischaemia in the brain and may act as neuroprotectants. In spinal cord, there is pharmacological evidence for increases in extracellular adenosine during hypoxia, but no direct measurements of purine release. Furthermore, the efflux pathways and origin of extracellular purines are not defined. To characterize hypoxia-evoked purine accumulation, we examined the effect of acute hypoxia on the extracellular levels of adenosine and inosine in isolated spinal cords from rats.

Experimental approach

Extracellular adenosine and inosine concentrations were assayed in an in vitro preparation of the isolated spinal cord of the neonatal rat by HPLC.

Key results

The extracellular level of inosin

SUBMITTER: Takahashi T 

PROVIDER: S-EPMC3010584 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature