Ontology highlight
ABSTRACT:
SUBMITTER: Tian Z
PROVIDER: S-EPMC3010896 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Proteomics 20101001 20
An online metal-free weak cation exchange-hydrophilic interaction LC/RPLC system has been developed for sensitive, high-throughput top-down MS. Here, we report results for analyzing PTMs of core histones, with a focus on histone H4, using this system. With just ∼24 μg on-column of core histones (H4, H2B, H2A, and H3) purified from human fibroblasts, 41 H4 isoforms were identified, with the type and location of PTMs unambiguously mapped for 20 of these variants. Compared to corresponding offline ...[more]