Transcriptional regulation of the hypocretin/orexin gene by NR6A1.
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ABSTRACT:
SUBMITTER: Tanaka S
PROVIDER: S-EPMC3012206 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
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Transcriptional regulation of the hypocretin/orexin gene by NR6A1.
Tanaka Susumu S Kodama Tohru T Nonaka Takashi T Toyoda Hiromi H Arai Makoto M Fukazawa Miyuki M Honda Yoshiko Y Honda Makoto M Mignot Emmanuel E
Biochemical and biophysical research communications 20101105 2
The hypocretin (also known as orexin) neuropeptide system coordinates the regulation of various physiological processes. A reduction in Nr6a1 expression was observed in hypocretin neuron-ablated transgenic mice. To show that prepro-hypocretin transcription is functionally modulated by NR6A1, we performed chromatin immunoprecipitation (ChIP) analysis, double-immunostaining, a luciferase reporter assay, and an in utero electroporation study. ChIP analysis showed that endogenous NR6A1 binds to a pu ...[more]
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