Ontology highlight
ABSTRACT:
SUBMITTER: Huovinen T
PROVIDER: S-EPMC3280210 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Huovinen Tuomas T Brockmann Eeva-Christine EC Akter Sultana S Perez-Gamarra Susan S Ylä-Pelto Jani J Liu Yuan Y Lamminmäki Urpo U
PloS one 20120215 2
Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A. Kunkel using uracil-containing single-stranded DNA as the template for the primer extension by additional uracil-DNA glycosylase treatment and rolling circle amplification (RCA) steps. It is shown that removal of uracil bases from the template leads to selective amplification of the nascently synthesized circular DNA strand ...[more]