Ontology highlight
ABSTRACT:
SUBMITTER: Hornung P
PROVIDER: S-EPMC3017645 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Hornung Peter P Maier Michael M Alushin Gregory M GM Lander Gabriel C GC Nogales Eva E Westermann Stefan S
Journal of molecular biology 20101112 2
Kinetochores are large multiprotein complexes that connect centromeres to spindle microtubules in all eukaryotes. Among the biochemically distinct kinetochore complexes, the conserved four-protein Mtw1 complex is a central part of the kinetochore in all organisms. Here we present the biochemical reconstitution and characterization of the budding yeast Mtw1 complex. Direct visualization by electron microscopy revealed an elongated bilobed structure with a 25-nm-long axis. The complex can be assem ...[more]