Ontology highlight
ABSTRACT:
SUBMITTER: Huntzinger E
PROVIDER: S-EPMC3018788 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Huntzinger Eric E Braun Joerg E JE Heimstädt Susanne S Zekri Latifa L Izaurralde Elisa E
The EMBO journal 20101109 24
miRNA-mediated gene silencing requires the GW182 proteins, which are characterized by an N-terminal domain that interacts with Argonaute proteins (AGOs), and a C-terminal silencing domain (SD). In Drosophila melanogaster (Dm) GW182 and a human (Hs) orthologue, TNRC6C, the SD was previously shown to interact with the cytoplasmic poly(A)-binding protein (PABPC1). Here, we show that two regions of GW182 proteins interact with PABPC1: the first contains a PABP-interacting motif 2 (PAM2; as shown bef ...[more]