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Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.

ABSTRACT: We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

SUBMITTER: Scarpellini P 

PROVIDER: S-EPMC89519 | biostudies-literature | 1999 Oct

REPOSITORIES: biostudies-literature

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Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis.

Scarpellini P P   Braglia S S   Carrera P P   Cedri M M   Cichero P P   Colombo A A   Crucianelli R R   Gori A A   Ferrari M M   Lazzarin A A  

Antimicrobial agents and chemotherapy 19991001 10

We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance. ...[more]

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