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Nucleoprotein-RNA orientation in the measles virus nucleocapsid by three-dimensional electron microscopy.

ABSTRACT: Recombinant measles virus nucleoprotein-RNA (N-RNA) helices were analyzed by negative-stain electron microscopy. Three-dimensional reconstructions of trypsin-digested and intact nucleocapsids coupled to the docking of the atomic structure of the respiratory syncytial virus (RSV) N-RNA subunit into the electron microscopy density map support a model that places the RNA at the exterior of the helix and the disordered C-terminal domain toward the helix interior, and they suggest the position of the six nucleotides with respect to the measles N protomer.

SUBMITTER: Desfosses A

PROVIDER: S-EPMC3020520 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Nucleoprotein-RNA orientation in the measles virus nucleocapsid by three-dimensional electron microscopy.

Desfosses Ambroise A Goret Gaël G Farias Estrozi Leandro L Ruigrok Rob W H RW Gutsche Irina I

Journal of virology 20101124 3

Recombinant measles virus nucleoprotein-RNA (N-RNA) helices were analyzed by negative-stain electron microscopy. Three-dimensional reconstructions of trypsin-digested and intact nucleocapsids coupled to the docking of the atomic structure of the respiratory syncytial virus (RSV) N-RNA subunit into the electron microscopy density map support a model that places the RNA at the exterior of the helix and the disordered C-terminal domain toward the helix interior, and they suggest the position of the ...[more]

PMID: 21106738

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