Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.
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ABSTRACT:
SUBMITTER: Roder B
PROVIDER: S-EPMC3020885 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.
Röder Barbara B Frühwirth Karin K Vogl Claus C Wagner Martin M Rossmanith Peter P
Journal of clinical microbiology 20100901 11
Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage. ...[more]
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