Ontology highlight
ABSTRACT:
SUBMITTER: Soriano Del Amo D
PROVIDER: S-EPMC3021957 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Soriano Del Amo David D Wang Wei W Jiang Hao H Besanceney Christen C Yan Amy C AC Levy Matthew M Liu Yi Y Marlow Florence L FL Wu Peng P
Journal of the American Chemical Society 20101109 47
The Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is the standard method for bioorthogonal conjugation. However, current Cu(I) catalyst formulations are toxic, hindering their use in living systems. Here we report that BTTES, a tris(triazolylmethyl)amine-based ligand for Cu(I), promotes the cycloaddition reaction rapidly in living systems without apparent toxicity. This catalyst allows, for the first time, noninvasive imaging of fucosylated glycans during zebrafish early embryogenesis. We m ...[more]