Ontology highlight
ABSTRACT:
SUBMITTER: Laughlin ST
PROVIDER: S-EPMC2701225 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Laughlin Scott T ST Baskin Jeremy M JM Amacher Sharon L SL Bertozzi Carolyn R CR
Science (New York, N.Y.) 20080501 5876
Glycans are attractive targets for molecular imaging but have been inaccessible because of their incompatibility with genetically encoded reporters. We demonstrated the noninvasive imaging of glycans in live developing zebrafish, using a chemical reporter strategy. Zebrafish embryos were treated with an unnatural sugar to metabolically label their cell-surface glycans with azides. Subsequently, the embryos were reacted with fluorophore conjugates by means of copper-free click chemistry, enabling ...[more]