Unknown

Dataset Information

0

Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors.


ABSTRACT: Here we describe the cloning and initial characterization of a previously unidentified CRF-related neuropeptide, urocortin II (Ucn II). Searches of the public human genome database identified a region with significant sequence homology to the CRF neuropeptide family. By using homologous primers deduced from the human sequence, a mouse cDNA was isolated from whole brain poly(A)(+) RNA that encodes a predicted 38-aa peptide, structurally related to the other known mammalian family members, CRF and Ucn. Ucn II binds selectively to the type 2 CRF receptor (CRF-R2), with no appreciable activity on CRF-R1. Transcripts encoding Ucn II are expressed in discrete regions of the rodent central nervous system, including stress-related cell groups in the hypothalamus (paraventricular and arcuate nuclei) and brainstem (locus coeruleus). Central administration of 1-10 microg of peptide elicits activational responses (Fos induction) preferentially within a core circuitry subserving autonomic and neuroendocrine regulation, but whose overall pattern does not broadly mimic the CRF-R2 distribution. Behaviorally, central Ucn II attenuates nighttime feeding, with a time course distinct from that seen in response to CRF. In contrast to CRF, however, central Ucn II failed to increase gross motor activity. These findings identify Ucn II as a new member of the CRF family of neuropeptides, which is expressed centrally and binds selectively to CRF-R2. Initial functional studies are consistent with Ucn II involvement in central autonomic and appetitive control, but not in generalized behavioral activation.

SUBMITTER: Reyes TM 

PROVIDER: S-EPMC30227 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors.

Reyes T M TM   Lewis K K   Perrin M H MH   Kunitake K S KS   Vaughan J J   Arias C A CA   Hogenesch J B JB   Gulyas J J   Rivier J J   Vale W W WW   Sawchenko P E PE  

Proceedings of the National Academy of Sciences of the United States of America 20010201 5


Here we describe the cloning and initial characterization of a previously unidentified CRF-related neuropeptide, urocortin II (Ucn II). Searches of the public human genome database identified a region with significant sequence homology to the CRF neuropeptide family. By using homologous primers deduced from the human sequence, a mouse cDNA was isolated from whole brain poly(A)(+) RNA that encodes a predicted 38-aa peptide, structurally related to the other known mammalian family members, CRF and  ...[more]

Similar Datasets

| S-EPMC2276373 | biostudies-literature
| S-EPMC34709 | biostudies-literature
| S-EPMC6155477 | biostudies-literature
| S-EPMC4006935 | biostudies-literature
| S-EPMC5959268 | biostudies-literature
| S-EPMC6729414 | biostudies-literature
| S-EPMC7061258 | biostudies-literature
| S-EPMC6632482 | biostudies-literature
| S-EPMC8234503 | biostudies-literature
| S-EPMC3129836 | biostudies-literature