Ontology highlight
ABSTRACT:
SUBMITTER: Gong XM
PROVIDER: S-EPMC3033194 | biostudies-literature | 2004 Jul
REPOSITORIES: biostudies-literature
Gong Xiao-Min XM Choi Jungyuen J Franzin Carla M CM Zhai Dayong D Reed John C JC Marassi Francesca M FM
The Journal of biological chemistry 20040428 28
The proapoptotic Bcl-2 family protein Bid is cleaved by caspase-8 to release the C-terminal fragment tBid, which translocates to the outer mitochondrial membrane and induces massive cytochrome c release and cell death. In this study, we have characterized the conformation of tBid in lipid membrane environments, using NMR and CD spectroscopy with lipid micelle and lipid bilayer samples. In micelles, tBid adopts a unique helical conformation, and the solution NMR (1)H/(15)N HSQC spectra have a sin ...[more]