Ontology highlight
ABSTRACT:
SUBMITTER: Sudhanshu B
PROVIDER: S-EPMC3033304 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Sudhanshu B B Mihardja S S Koslover E F EF Mehraeen S S Bustamante C C Spakowitz A J AJ
Proceedings of the National Academy of Sciences of the United States of America 20110118 5
We analyze the response of a single nucleosome to tension, which serves as a prototypical biophysical measurement where tension-dependent deformation alters transition kinetics. We develop a statistical-mechanics model of a nucleosome as a wormlike chain bound to a spool, incorporating fluctuations in the number of bases bound, the spool orientation, and the conformations of the unbound polymer segments. With the resulting free-energy surface, we perform dynamic simulations that permit a direct ...[more]