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MS-1020 is a novel small molecule that selectively inhibits JAK3 activity.


ABSTRACT: In order to identify Janus kinase/signal transducer and activator of transcription (JAK/STAT) signalling inhibitors, a cell-based high throughput screening was performed using a plant extract library that identified Nb-(alpha-hydroxynaphthoyl)serotonin called MS-1020 as a novel JAK3 inhibitor. MS-1020 potently inhibited persistently-active STAT3 in a cell type-specific manner. Further examination showed that MS-1020 selectively blocked constitutively-active JAK3 and consistently suppressed interleukin-2-induced JAK3/STAT5 signalling but not prolactin-induced JAK2/STAT5 signalling. Furthermore, MS-1020 affected cell viability only in cancer cells harbouring persistently-active JAK3/STATs, and in vitro kinase assays showed MS-1020 binds directly with JAK3, blocking its catalytic activity. Therefore, the present study suggested that this reagent selectively inhibits JAK3 and subsequently leads to a block in STAT signalling. Finally, MS-1020 decreased cell survival by inducing apoptosis via down-regulation of anti-apoptotic gene expression. These results suggest that MS-1020 may have therapeutic potential in the treatment of cancers harbouring aberrant JAK3 signalling.

SUBMITTER: Kim BH 

PROVIDER: S-EPMC3034357 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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MS-1020 is a novel small molecule that selectively inhibits JAK3 activity.

Kim Byung-Hak BH   Oh Sei-Ryang SR   Yin Chang-Hong CH   Lee Sangku S   Kim Eun-Ah EA   Kim Min-Seok MS   Sandoval Claudio C   Jayabose Somasundaram S   Bach Erika A EA   Lee Hyeong-Kyu HK   Baeg Gyeong-Hun GH  

British journal of haematology 20090929 1


In order to identify Janus kinase/signal transducer and activator of transcription (JAK/STAT) signalling inhibitors, a cell-based high throughput screening was performed using a plant extract library that identified Nb-(alpha-hydroxynaphthoyl)serotonin called MS-1020 as a novel JAK3 inhibitor. MS-1020 potently inhibited persistently-active STAT3 in a cell type-specific manner. Further examination showed that MS-1020 selectively blocked constitutively-active JAK3 and consistently suppressed inter  ...[more]

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