Ontology highlight
ABSTRACT:
SUBMITTER: Beebe ET
PROVIDER: S-EPMC3035758 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Beebe Emily T ET Makino Shin-Ichi S Nozawa Akira A Matsubara Yuko Y Frederick Ronnie O RO Primm John G JG Goren Michael A MA Fox Brian G BG
New biotechnology 20100715 3
The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported. The soluble Galdieria sulphuraria protein DCN1 was obtained in greater than 2mg total synthesis yield per mL of reaction mixture from the Protemist XE, and the structure was subsequently solved by X-ray crystallography using material from one 10 mL synthesis (PDB ID: 3KEV). The Protemist XE was also capable of membrane protein translation. Thus human sigma-1 receptor was t ...[more]