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Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene.


ABSTRACT: Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.

SUBMITTER: Chen C 

PROVIDER: S-EPMC3036127 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene.

Chen Congying C   Sargent Carole C   Quilter Claire C   Yang Zhuqing Z   Ren Jun J   Affara Nabeel N   Brenig Bertram B   Huang Lusheng L  

Genetics and molecular biology 20100701 3


Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein o  ...[more]

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