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ABSTRACT:
SUBMITTER: Bartolommei G
PROVIDER: S-EPMC3042568 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Bartolommei Gianluca G Gramigni Elisa E Tadini-Buoninsegni Francesco F Santini Giacomo G Moncelli Maria Rosa MR
Biophysical journal 20101001 7
The effect of Pb(2+) ions on the Na(+),K(+)-ATPase was investigated in detail by means of steady-state fluorescence spectroscopy. Experiments were performed by using the electrochromic styryl dye RH421. It is shown that Pb(2+) ions can bind reversibly to the protein and do not affect the Na(+) and K(+) binding affinities in the E(1) and P-E(2) conformations of the enzyme. The pH titrations indicate that lead(II) favors binding of one H(+) to the P-E(2) conformation in the absence of K(+). A mode ...[more]