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Polymerized planar suspended lipid bilayers for single ion channel recordings: comparison of several dienoyl lipids.


ABSTRACT: The stabilization of suspended planar lipid membranes, or black lipid membranes (BLMs), through polymerization of mono- and bis-functionalized dienoyl lipids was investigated. Electrical properties, including capacitance, conductance, and dielectric breakdown voltage, were determined for BLMs composed of mono-DenPC, bis-DenPC, mono-SorbPC, and bis-SorbPC both prior to and following photopolymerization, with diphytanoyl phosphocholine (DPhPC) serving as a control. Poly(lipid) BLMs exhibited significantly longer lifetimes and increased the stability of air-water transfers. BLM stability followed the order bis-DenPC > mono-DenPC ? mono-SorbPC > bis-SorbPC. The conductance of bis-SorbPC BLMs was significantly higher than that of the other lipids, which is attributed to a high density of hydrophilic pores, resulting in relatively unstable membranes. The use of poly(lipid) BLMs as matrices for supporting the activity of an ion channel protein (IC) was explored using ?-hemolysin (?-HL), a model IC. Characteristic i-V plots of ?-HL were maintained following photopolymerization of bis-DenPC, mono-DenPC, and mono-SorbPC, demonstrating the utility of these materials for preparing more durable BLMs for single-channel recordings of reconstituted ICs.

SUBMITTER: Heitz BA 

PROVIDER: S-EPMC3043114 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Polymerized planar suspended lipid bilayers for single ion channel recordings: comparison of several dienoyl lipids.

Heitz Benjamin A BA   Xu Juhua J   Jones Ian W IW   Keogh John P JP   Comi Troy J TJ   Hall Henry K HK   Aspinwall Craig A CA   Saavedra S Scott SS  

Langmuir : the ACS journal of surfaces and colloids 20110112 5


The stabilization of suspended planar lipid membranes, or black lipid membranes (BLMs), through polymerization of mono- and bis-functionalized dienoyl lipids was investigated. Electrical properties, including capacitance, conductance, and dielectric breakdown voltage, were determined for BLMs composed of mono-DenPC, bis-DenPC, mono-SorbPC, and bis-SorbPC both prior to and following photopolymerization, with diphytanoyl phosphocholine (DPhPC) serving as a control. Poly(lipid) BLMs exhibited signi  ...[more]

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