Generation of viable male and female mice from two fathers.
Ontology highlight
ABSTRACT:
SUBMITTER: Deng JM
PROVIDER: S-EPMC3043133 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Publications
Generation of viable male and female mice from two fathers.
Deng Jian Min JM Satoh Kei K Wang Hongran H Chang Hao H Zhang Zhaoping Z Stewart M David MD Cooney Austin J AJ Behringer Richard R RR
Biology of reproduction 20101208 3
In sexual species, fertilization of oocytes produces individuals with alleles derived from both parents. Here we use pluripotent stem cells derived from somatic cells to combine the haploid genomes from two males to produce viable sons and daughters. Male (XY) mouse induced pluripotent stem cells (Father #1) were used to isolate subclones that had spontaneously lost the Y chromosome to become genetically female (XO). These male-derived XO stem cells were used to generate female chimeras that wer ...[more]
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