Ontology highlight
ABSTRACT:
SUBMITTER: Liu C
PROVIDER: S-EPMC3043212 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Biophysical journal 20110301 5
Speed and processivity of replicative DNA polymerases can be enhanced via coupling to a sliding clamp. Due to the closed ring shape of the clamp, a clamp loader protein, belonging to the AAA+ class of ATPases, needs to open the ring-shaped clamp before loading it to DNA. Here, we developed real-time fluorescence assays to study the clamp (PCNA) and the clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans. Unexpectedly, we discovered that RFC can assemble a PCNA ring from mo ...[more]