Ontology highlight
ABSTRACT:
SUBMITTER: Balamurugan A
PROVIDER: S-EPMC3043618 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Balamurugan Arumugam A Ng Hwee L HL Yang Otto O OO
Journal of immunology (Baltimore, Md. : 1950) 20101013 10
TCRs mediate CTL specificity, but TCRs recognizing the same epitope often differ between persons due to their stochastic derivation. The role of this variability in the pathogenesis of virus infections and malignancies has been technically difficult to study. We apply an adaptation of TCR spectratyping to study HIV-specific CTLs, defining the clonal breadth and sequences of epitope-specific TCRs from PBMCs without cellular sorting or molecular cloning. Examining 48 CTL responses in 12 persons re ...[more]