Ontology highlight
ABSTRACT:
SUBMITTER: Gajewski C
PROVIDER: S-EPMC3044366 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Gajewski Christine C Dagcan Alper A Roux Benoit B Deutsch Carol C
Proceedings of the National Academy of Sciences of the United States of America 20110207 8
The pore domain of voltage-gated potassium (Kv) channels consists of transmembrane helices S5 and S6, the turret, the pore helix, the selectivity filter, and the loop preceding S6, with a tertiary reentrant structure between S5 and S6. Using biogenic intermediates, mass tagging (pegylation), and a molecular tape measure, we explored the possibility that the first stages of pore formation occur prior to oligomerization of the transmembrane core. Pegylation of introduced cysteines shows that the p ...[more]