Ontology highlight
ABSTRACT:
SUBMITTER: Gerbasi VR
PROVIDER: S-EPMC3044420 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Gerbasi Vincent R VR Preall Jonathan B JB Golden Daniel E DE Powell David W DW Cummins Timothy D TD Sontheimer Erik J EJ
Proceedings of the National Academy of Sciences of the United States of America 20110207 8
Small RNAs and a diverse array of protein partners control gene expression in eukaryotes through a variety of mechanisms. By combining siRNA affinity chromatography and mass spectrometry, we have identified the double-stranded RNA-binding domain protein Blanks to be an siRNA- and dsRNA-binding protein from Drosophila S2 cells. We find that Blanks is a nuclear factor that contributes to the efficiency of RNAi. Biochemical fractionation of a Blanks-containing complex shows that the Blanks complex ...[more]