MicroRNA-203 inhibits cell proliferation by repressing ?Np63 expression in human esophageal squamous cell carcinoma.

ABSTRACT:

Background

This study was performed to investigate the effect of microRNA-203 (miR-203) and ?Np63 on cell proliferation and the functional connection between miR-203 and ?Np63 in ESCC.

Methods

We employed 2 human ESCC cell lines, Eca109 and TE-1, as the model system. The effect of miR-203 and ?Np63 on cell proliferation was determined in cells transfected with miR-203 mimic and ?Np63 small interfering RNA (siRNA), respectively. The regulation of ?Np63 expression in ESCC cells by miR-203 was studied by luciferase reporter assay, RT-PCR and western blot analysis in cells transfected with miR-203. The effect of ?Np63 re-expression on miR-203 induced inhibition of cell proliferation was studied by cell proliferation assay in cells cotransfected with miR-203 and pcDNA-?Np63 plasmid (without the 3'-UTR of ?Np63).

Results

We found that both miR-203 and ?Np63 siRNA signicantly inhibited cell proliferation in ESCC. MiR-203 could down-regulate endogenous ?Np63 expression at the posttranscriptional level. Moreover, re-expression of ?Np63 in cells transfected with miR-203 significantly attenuated the miR-203 induced inhibition of cell proliferation.

Conclusions

Our data implied that miR-203 could inhibit cell proliferation in human ESCC through ?Np63-mediated signal pathway. Therefore, we propose that miR-203 might be used as a therapeutic agent for human ESCC.

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC3044653 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature