Ontology highlight
ABSTRACT:
SUBMITTER: Leduc F
PROVIDER: S-EPMC3045442 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Leduc Frédéric F Faucher David D Bikond Nkoma Geneviève G Grégoire Marie-Chantal MC Arguin Mélina M Wellinger Raymund J RJ Boissonneault Guylain G
PloS one 20110225 2
Determination of cellular DNA damage has so far been limited to global assessment of genome integrity whereas nucleotide-level mapping has been restricted to specific loci by the use of specific primers. Therefore, only limited DNA sequences can be studied and novel regions of genomic instability can hardly be discovered. Using a well-characterized yeast model, we describe a straightforward strategy to map genome-wide DNA strand breaks without compromising nucleotide-level resolution. This techn ...[more]