Ontology highlight
ABSTRACT:
SUBMITTER: Petrosino G
PROVIDER: S-EPMC7580242 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
STAR protocols 20200805 2
GLOE-Seq is a next-generation sequencing method for the genome-wide mapping of 3'-OH termini, either resulting from single- or double-strand breaks or introduced by enzymatic conversion of lesions or modified nucleotides. This protocol provides instructions for isolation of genomic DNA from budding yeast or mammalian cells, preparation of libraries for sequencing, and data analysis by the associated computational pipeline, GLOE-Pipe. It is optimized for the Illumina next-generation sequencing pl ...[more]