Biochips that sequentially capture and focus antigens for immunoaffinity MALDI-TOF MS: a new tool for biomarker verification.
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ABSTRACT:
SUBMITTER: Brauer HA
PROVIDER: S-EPMC3046459 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Biochips that sequentially capture and focus antigens for immunoaffinity MALDI-TOF MS: a new tool for biomarker verification.
Brauer Heather Ann HA Lampe Paul D PD Yasui Yutaka Y YY Hamajima Nobuyuki N Stolowitz Mark L ML
Proteomics 20101101 21
A novel approach to immunoaffinity MS is described wherein antibodies are appended to a patterned gold Biochip surface. The Biochip surface is patterned with an array of concentric immunocapture zones composed of highly hydrophilic central zones surrounded by moderately hydrophilic zones that reside on a non-wetting background, with protein attachment via electrochemically cleavable linkers. After linker cleavage, matrix application forms a discrete spot suitable for MALDI-TOF-MS. Use of the Bio ...[more]
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