Ontology highlight
ABSTRACT:
SUBMITTER: Adey A
PROVIDER: S-EPMC3046479 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Adey Andrew A Morrison Hilary G HG Asan Xun Xu X Kitzman Jacob O JO Turner Emily H EH Stackhouse Bethany B MacKenzie Alexandra P AP Caruccio Nicholas C NC Zhang Xiuqing X Shendure Jay J
Genome biology 20101208 12
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library constr ...[more]