Ontology highlight
ABSTRACT:
SUBMITTER: Choi HM
PROVIDER: S-EPMC3058322 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Choi Harry M T HM Chang Joann Y JY Trinh Le A le A Padilla Jennifer E JE Fraser Scott E SE Pierce Niles A NA
Nature biotechnology 20101031 11
In situ hybridization methods enable the mapping of mRNA expression within intact biological samples. With current approaches, it is challenging to simultaneously map multiple target mRNAs within whole-mount vertebrate embryos, representing a significant limitation in attempting to study interacting regulatory elements in systems most relevant to human development and disease. Here, we report a multiplexed fluorescent in situ hybridization method based on orthogonal amplification with hybridizat ...[more]