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Escherichia coli RNA polymerase core and holoenzyme structures.

ABSTRACT: Multisubunit RNA polymerase is an essential enzyme for regulated gene expression. Here we report two Escherichia coli RNA polymerase structures: an 11.0 A structure of the core RNA polymerase and a 9.5 A structure of the sigma(70) holoenzyme. Both structures were obtained by cryo-electron microscopy and angular reconstitution. Core RNA polymerase exists in an open conformation. Extensive conformational changes occur between the core and the holoenzyme forms of the RNA polymerase, which are largely associated with movements in ss'. All common RNA polymerase subunits (alpha(2), ss, ss') could be localized in both structures, thus suggesting the position of sigma(70) in the holoenzyme.

SUBMITTER: Finn RD

PROVIDER: S-EPMC305883 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature

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Escherichia coli RNA polymerase core and holoenzyme structures.

Finn R D RD Orlova E V EV Gowen B B Buck M M van Heel M M

The EMBO journal 20001201 24

Multisubunit RNA polymerase is an essential enzyme for regulated gene expression. Here we report two Escherichia coli RNA polymerase structures: an 11.0 A structure of the core RNA polymerase and a 9.5 A structure of the sigma(70) holoenzyme. Both structures were obtained by cryo-electron microscopy and angular reconstitution. Core RNA polymerase exists in an open conformation. Extensive conformational changes occur between the core and the holoenzyme forms of the RNA polymerase, which are large ...[more]

PMID: 11118218

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