Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe S
PROVIDER: S-EPMC3059187 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Watanabe Shigeki S Punge Annedore A Hollopeter Gunther G Willig Katrin I KI Hobson Robert John RJ Davis M Wayne MW Hell Stefan W SW Jorgensen Erik M EM
Nature methods 20101121 1
A complete portrait of a cell requires a detailed description of its molecular topography: proteins must be linked to particular organelles. Immunocytochemical electron microscopy can reveal locations of proteins with nanometer resolution but is limited by the quality of fixation, the paucity of antibodies and the inaccessibility of antigens. Here we describe correlative fluorescence electron microscopy for the nanoscopic localization of proteins in electron micrographs. We tagged proteins with ...[more]