Ontology highlight
ABSTRACT:
SUBMITTER: Huang F
PROVIDER: S-EPMC3696415 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Nature methods 20130526 7
Newly developed scientific complementary metal-oxide semiconductor (sCMOS) cameras have the potential to dramatically accelerate data acquisition, enlarge the field of view and increase the effective quantum efficiency in single-molecule switching nanoscopy. However, sCMOS-intrinsic pixel-dependent readout noise substantially lowers the localization precision and introduces localization artifacts. We present algorithms that overcome these limitations and that provide unbiased, precise localizati ...[more]