Unknown

Dataset Information

0

The mouse hairy ears mutation exhibits an extended growth (anagen) phase in hair follicles and altered Hoxc gene expression in the ears.


ABSTRACT: The mouse In(15)2Rl (hairy ears, Eh) mutation is a paracentric inversion of the distal half of chromosome 15 (Chr 15). Heterozygous Eh/+ mice display misshaped and hairy ears that have more and longer hair than the ears of their wild-type littermates. We mapped, cloned and sequenced both inversion breakpoints. No protein-coding transcript was disrupted by either breakpoint. The proximal breakpoint is located between syntrophin basic 1 (Sntb1) and hyaluronan synthase 2 (Has2), and the distal breakpoint maps between homeobox C4 (Hoxc4) and single-strand selective monofunctional uracil DNA glycosylase (Smug1), near the middle and the telomere ends of Chr 15, respectively. The inversion spans ~47 megabases. Our genetic analysis suggests that the hairy-ear phenotype is caused by the proximal breakpoint of the inversion-bearing Chr 15. Quantitative RNA analysis by real-time polymerase chain reaction for the genes flanking the breakpoint indicated no changes in expression levels except for some homeobox C (Hoxc) genes whose expression was elevated in developing and mature skin of the ears but not of other body regions. The increased hair length on the ears of Eh/+ mice was due to an extension of the anagen stage in the hair cycle, as determined by histological analysis. Our data indicate that the Eh phenotype arises from mis-expression of Hoxc genes.

SUBMITTER: Mentzer SE 

PROVIDER: S-EPMC3061202 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

The mouse hairy ears mutation exhibits an extended growth (anagen) phase in hair follicles and altered Hoxc gene expression in the ears.

Mentzer Sarah E SE   Sundberg John P JP   Awgulewitsch Alexander A   Chao Hanna H J HH   Carpenter Donald A DA   Zhang Wei-Dong WD   Rinchik Eugene M EM   You Yun Y  

Veterinary dermatology 20081114 6


The mouse In(15)2Rl (hairy ears, Eh) mutation is a paracentric inversion of the distal half of chromosome 15 (Chr 15). Heterozygous Eh/+ mice display misshaped and hairy ears that have more and longer hair than the ears of their wild-type littermates. We mapped, cloned and sequenced both inversion breakpoints. No protein-coding transcript was disrupted by either breakpoint. The proximal breakpoint is located between syntrophin basic 1 (Sntb1) and hyaluronan synthase 2 (Has2), and the distal brea  ...[more]

Similar Datasets

| S-EPMC9822907 | biostudies-literature
| S-EPMC4849038 | biostudies-literature
| S-EPMC6385416 | biostudies-literature
| S-EPMC3675505 | biostudies-literature
| S-EPMC7720164 | biostudies-literature
| S-EPMC7463739 | biostudies-literature
2009-03-25 | E-GEOD-15185 | biostudies-arrayexpress
| S-EPMC2583201 | biostudies-other
| S-EPMC2765465 | biostudies-literature
| S-EPMC9566782 | biostudies-literature