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Endocytosis regulates cell soma translocation and the distribution of adhesion proteins in migrating neurons.

ABSTRACT: Newborn neurons migrate from their birthplace to their final location to form a properly functioning nervous system. During these movements, young neurons must attach and subsequently detach from their substrate to facilitate migration, but little is known about the mechanisms cells use to release their attachments. We show that the machinery for clathrin-mediated endocytosis is positioned to regulate the distribution of adhesion proteins in a subcellular region just proximal to the neuronal cell body. Inhibiting clathrin or dynamin function impedes the movement of migrating neurons both in vitro and in vivo. Inhibiting dynamin function in vitro shifts the distribution of adhesion proteins to the rear of the cell. These results suggest that endocytosis may play a critical role in regulating substrate detachment to enable cell body translocation in migrating neurons.

SUBMITTER: Shieh JC

PROVIDER: S-EPMC3062553 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Endocytosis regulates cell soma translocation and the distribution of adhesion proteins in migrating neurons.

Shieh Jennifer C JC Schaar Bruce T BT Srinivasan Karpagam K Brodsky Frances M FM McConnell Susan K SK

PloS one 20110322 3

Newborn neurons migrate from their birthplace to their final location to form a properly functioning nervous system. During these movements, young neurons must attach and subsequently detach from their substrate to facilitate migration, but little is known about the mechanisms cells use to release their attachments. We show that the machinery for clathrin-mediated endocytosis is positioned to regulate the distribution of adhesion proteins in a subcellular region just proximal to the neuronal cel ...[more]

PMID: 21445347

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