Ontology highlight
ABSTRACT:
SUBMITTER: Zhai B
PROVIDER: S-EPMC3063950 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Zhai Bo B Villén Judit J Beausoleil Sean A SA Mintseris Julian J Gygi Steven P SP
Journal of proteome research 20080308 4
Protein phosphorylation is a key regulatory event in most cellular processes and development. Mass spectrometry-based proteomics provides a framework for the large-scale identification and characterization of phosphorylation sites. Here, we used a well-established phosphopeptide enrichment and identification strategy including the combination of strong cation exchange chromatography, immobilized metal affinity chromatography, and high-accuracy mass spectrometry instrumentation to study phosphory ...[more]