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Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation.

ABSTRACT: mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.

SUBMITTER: Fu J

PROVIDER: S-EPMC3064370 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation.

Fu Jie J Munro James B JB Blanchard Scott C SC Frank Joachim J

Proceedings of the National Academy of Sciences of the United States of America 20110307 12

mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated. ...[more]

PMID: 21383139

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