Ontology highlight
ABSTRACT:
SUBMITTER: van Oeveren J
PROVIDER: S-EPMC3065709 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
van Oeveren Jan J de Ruiter Marjo M Jesse Taco T van der Poel Hein H Tang Jifeng J Yalcin Feyruz F Janssen Antoine A Volpin Hanne H Stormo Keith E KE Bogden Robert R van Eijk Michiel J T MJ Prins Marcel M
Genome research 20110201 4
We present whole genome profiling (WGP), a novel next-generation sequencing-based physical mapping technology for construction of bacterial artificial chromosome (BAC) contigs of complex genomes, using Arabidopsis thaliana as an example. WGP leverages short read sequences derived from restriction fragments of two-dimensionally pooled BAC clones to generate sequence tags. These sequence tags are assigned to individual BAC clones, followed by assembly of BAC contigs based on shared regions contain ...[more]