Ontology highlight
ABSTRACT:
SUBMITTER: Puckett JW
PROVIDER: S-EPMC3066056 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Puckett James W JW Muzikar Katy A KA Tietjen Josh J Warren Christopher L CL Ansari Aseem Z AZ Dervan Peter B PB
Journal of the American Chemical Society 20070919 40
A high-throughput Cognate Site Identity (CSI) microarray platform interrogating all 524 800 10-base pair variable sites is correlated to quantitative DNase I footprinting data of DNA binding pyrrole-imidazole polyamides. An eight-ring hairpin polyamide programmed to target the 5 bp sequence 5'-TACGT-3' within the hypoxia response element (HRE) yielded a CSI microarray-derived sequence motif of 5'-WWACGT-3' (W = A,T). A linear beta-linked polyamide programmed to target a (GAA)3 repeat yielded a C ...[more]