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Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene.

ABSTRACT: The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, but established between 1.5 d.p.c. and 12.5 d.p.c. on the contrary, the allele-specific expression occurred in the two-cell stage when transcription initiates. Absence of the methylation of the upstream region in this stage indicates that methylation is not necessary for inactivation of the expression.

SUBMITTER: Hatada I

PROVIDER: S-EPMC306627 | biostudies-literature | 1995 Jan

REPOSITORIES: biostudies-literature

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Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene.

Hatada I I Kitagawa K K Yamaoka T T Wang X X Arai Y Y Hashido K K Ohishi S S Masuda J J Ogata J J Mukai T T

Nucleic acids research 19950101 1

The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, ...[more]

PMID: 7870588

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