Ontology highlight
ABSTRACT:
SUBMITTER: Lee CH
PROVIDER: S-EPMC3069399 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Lee Chien-Hsien CH Pan Yih-Jiuan YJ Huang Yun-Tzu YT Liu Tseng-Huang TH Hsu Shen-Hsing SH Lee Ching-Hung CH Chen Yen-Wei YW Lin Shih-Ming SM Huang Lin-Kun LK Pan Rong-Long RL
The Journal of biological chemistry 20110203 14
H+-translocating pyrophosphatase (H+-PPase; EC 3.6.1.1) drives proton transport against an electrochemical potential gradient by hydrolyzing pyrophosphate (PPi) and is found in various endomembranes of higher plants, bacteria, and some protists. H+-PPase contains seven highly conserved lysines. We examined the functional roles of these lysines, which are, for the most part, found in the cytosolic regions of mung bean H+-PPase by site-directed mutagenesis. Construction of mutants that each had a ...[more]