Ontology highlight
ABSTRACT:
SUBMITTER: Koornneef A
PROVIDER: S-EPMC3070114 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Koornneef Annemart A Maczuga Piotr P van Logtenstein Richard R Borel Florie F Blits Bas B Ritsema Tita T van Deventer Sander S Petry Harald H Konstantinova Pavlina P
Molecular therapy : the journal of the American Society of Gene Therapy 20110208 4
Serum low-density lipoprotein cholesterol (LDL-C) levels are proportionate to the risk of atherosclerotic cardiovascular disease. In order to reduce serum total cholesterol and LDL-C levels in mice, RNA interference (RNAi) was used to inhibit expression of the structural protein of LDL-C, apolipoprotein B100 (ApoB). We developed and screened 19 short hairpin RNAs (shRNAs) targeting conserved sequences in human, mouse, and macaque ApoB mRNAs (shApoB) and subsequently narrowed our focus to one can ...[more]