Next-generation high-density self-assembling functional protein arrays.
Ontology highlight
ABSTRACT:
SUBMITTER: Ramachandran N
PROVIDER: S-EPMC3070491 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Publications
Next-generation high-density self-assembling functional protein arrays.
Ramachandran Niroshan N Raphael Jacob V JV Hainsworth Eugenie E Demirkan Gokhan G Fuentes Manuel G MG Rolfs Andreas A Hu Yanhui Y LaBaer Joshua J
Nature methods 20080511 6
We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput. ...[more]
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