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The nuts and bolts of ring-translocase structure and mechanism.

ABSTRACT: Ring-shaped, oligomeric translocases are multisubunit enzymes that couple the hydrolysis of Nucleoside TriPhosphates (NTPs) to directed movement along extended biopolymer substrates. These motors help unwind nucleic acid duplexes, unfold protein chains, and shepherd nucleic acids between cellular and/or viral compartments. Substrates are translocated through a central pore formed by a circular array of catalytic subunits. Cycles of nucleotide binding, hydrolysis, and product release help reposition translocation loops in the pore to direct movement. How NTP turnover allosterically induces these conformational changes, and the extent of mechanistic divergence between motor families, remain outstanding problems. This review examines the current models for ring-translocase function and highlights the fundamental gaps remaining in our understanding of these molecular machines.

SUBMITTER: Lyubimov AY

PROVIDER: S-EPMC3070846 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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The nuts and bolts of ring-translocase structure and mechanism.

Lyubimov Artem Y AY Strycharska Melania M Berger James M JM

Current opinion in structural biology 20110201 2

Ring-shaped, oligomeric translocases are multisubunit enzymes that couple the hydrolysis of Nucleoside TriPhosphates (NTPs) to directed movement along extended biopolymer substrates. These motors help unwind nucleic acid duplexes, unfold protein chains, and shepherd nucleic acids between cellular and/or viral compartments. Substrates are translocated through a central pore formed by a circular array of catalytic subunits. Cycles of nucleotide binding, hydrolysis, and product release help reposit ...[more]

PMID: 21282052

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The nuts and bolts of ring-translocase structure and mechanism.

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The nuts and bolts of ring-translocase structure and mechanism.

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