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Protein-ligand binding investigated by a single nanoparticle TERS approach.

ABSTRACT: We report TERS imaging of individual 50 nm, biotin-labeled gold nanoparticles bound to a streptavidin-derivatized glass slide. Individual gold nanoparticles detected by a nanoparticle TERS tip generate Raman enhancements in both the biotin and streptavidin signals. These results indicate that nanoparticles are capable of investigating nanoscale spatial and chemical environments with non-resonant Raman enhancements.

SUBMITTER: Carrier SL

PROVIDER: S-EPMC3072886 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Protein-ligand binding investigated by a single nanoparticle TERS approach.

Carrier Stacey L SL Kownacki Corey M CM Schultz Zachary D ZD

Chemical communications (Cambridge, England) 20110104 7

We report TERS imaging of individual 50 nm, biotin-labeled gold nanoparticles bound to a streptavidin-derivatized glass slide. Individual gold nanoparticles detected by a nanoparticle TERS tip generate Raman enhancements in both the biotin and streptavidin signals. These results indicate that nanoparticles are capable of investigating nanoscale spatial and chemical environments with non-resonant Raman enhancements. ...[more]

PMID: 21206948

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