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High-throughput single-molecule optofluidic analysis.

ABSTRACT: We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

SUBMITTER: Kim S

PROVIDER: S-EPMC3075913 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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High-throughput single-molecule optofluidic analysis.

Kim Soohong S Streets Aaron M AM Lin Ron R RR Quake Stephen R SR Weiss Shimon S Majumdar Devdoot S DS

Nature methods 20110206 3

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment. ...[more]

PMID: 21297618

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