Ontology highlight
ABSTRACT:
SUBMITTER: Ingargiola A
PROVIDER: S-EPMC5395192 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Ingargiola Antonino A Lerner Eitan E Chung SangYoon S Panzeri Francesco F Gulinatti Angelo A Rech Ivan I Ghioni Massimo M Weiss Shimon S Michalet Xavier X
PloS one 20170418 4
We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be properly corrected and result in measured sample characteristics consistent with those obtained with a standard single-spot setup. We then take advantage of the higher throughput provided by parallel ...[more]